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GPR32

Family: Class A Orphans

Contents:
Gene and Protein Information
Previous and Unofficial Names
Database Links
Agonists
Tissue Distribution
Physiological Consequences of Altering Gene Expression
Clinically-Relevant Mutations and Pathophysiology
General Comments
References
Gene and Protein Information
class A G protein-coupled receptor
Species TM AA Chromosomal Location Gene Symbol Gene Name Reference
Human 7 356 19q13.3 GPR32 G protein-coupled receptor 32
Previous and Unofficial Names
GPR32
G-protein coupled receptor 32
probable G-protein coupled receptor 32
RvD1
RVDR1
resolvin D1 receptor
Database Links
Ensembl ENSG00000142511 (Hs)
Entrez Gene 2854 (Hs)
GeneCards GPR32 (Hs)
HomoloGene 88647 (Hs)
Human Protein Reference Database 04430 (Hs)
InterPro O75388 (Hs)
KEGG Gene hsa:2854 (Hs)
OMIM 603195 (Hs)
PharmGKB Gene PA28875 (Hs)
Protein Ontology (PRO) PRO:000001528 (Hs)
RefSeq Nucleotide NM_001506 (Hs)
RefSeq Protein NP_001497 (Hs)
TreeFam ENSG00000142511 (Hs)
UniGene Hs. 515555 (Hs)
UniProt O75388 (Hs)
Wikipedia GPR32
Orphan and other 7TM receptors, Resolvin receptors
Natural/Endogenous Ligand(s)
resolvin D1
Comments: Proposed ligand, single publication
Rank order of potency
resolvin D1 > LXA4
Agonist Comments
Resolvin D5 has been demonstrated to activate GPR32 in a single publication [2]. This result is supported by the findings of Chiang et al. which demonstrate that Resolvin D1 inhibits TNFalpha mediated NFκB activation via GPR32 [1].
Tissue Distribution
Myeloid cells, human umbilical vein cells
Species:  Human
Technique:  RT-PCR
References:  2
Arterial and venous tissue
Species:  Human
Technique:  Microarray analysis
References:  2
Tissue Distribution Comments
Surface expression in human monocytes up-regulated by zymosan and G-CSF [2].
Physiological Consequences of Altering Gene Expression
Selective knockdown decreases RvD1 mediate macrophage phagocytosis of human polymorphonuclear leukocytes
Species:  Human
Tissue:  Macrophage
Technique:  RNA interference
References:  2
Macrophages overexpressing GPR32 show significantly upregulated miRNA-21, amd downregulation of miR-302d and miR-219
Species:  Mouse
Tissue:  Macrophage
Technique:  Gene overexpression
References:  4
Overexpression of GPR32 enhances RvD1 mediated macrophage phagocytosis of human polymorphonuclear leukocytes
Species:  Human
Tissue:  Macrophage
Technique:  RNA interference
References:  2
Clinically-Relevant Mutations and Pathophysiology
Comments:  Resolvin mediated activation of GPR32 may be important in the resolution of inflammatory disease.
References:  3
Mutations not determined
General Comments
GPR32 is a pseudogene in mice and rats [2].
Available Assays
DiscoveRx PathHunter® CHO-K1 GPR32 (Orphan) High Expression β-Arrestin Cell Line Human Cat No. 93-0321C2A
DiscoveRx PathHunter® eXpress GPR32 CHO-K1 β-Arrestin (Orphan) GPCR Assay Human Cat No. 93-0321E2ACP2

REFERENCES

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1. Chiang, N; Fredman, G; Bäckhed, F; Oh, SF; Vickery, T; Schmidt, BA; Serhan, CN. (2012) Infection regulates pro-resolving mediators that lower antibiotic requirements. Nature, 484 (7395): 524-8. [PMID:22538616]

2. Krishnamoorthy, S; Recchiuti, A; Chiang, N; Yacoubian, S; Lee, CH; Yang, R; Petasis, NA; Serhan, CN. (2010) Resolvin D1 binds human phagocytes with evidence for proresolving receptors. Proc. Natl. Acad. Sci. U.S.A., 107 (4): 1660-5. [PMID:20080636]

3. Lee, CH. (2012) Resolvins as new fascinating drug candidates for inflammatory diseases. Arch. Pharm. Res., 35 (1): 3-7. [PMID:22297737]

4. Pappo, AS; Krailo, M; Chen, Z; Rodriguez-Galindo, C; Reaman, G. (2010) Infrequent tumor initiative of the Children's Oncology Group: initial lessons learned and their impact on future plans. J. Clin. Oncol., 28 (33): 5011-6. [PMID:20956621]

To cite this database page, please use the following:

Amy E. Monaghan.
Class A Orphans: GPR32. Last modified on 13/12/2012. Accessed on 25/05/2013. IUPHAR database (IUPHAR-DB), http://iuphar-db.org/DATABASE/ObjectDisplayForward?objectId=99.


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