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Kv2.2

Family: Voltage-gated potassium channels

Contents:
Gene and Protein Information
Previous and Unofficial Names
Database Links
Associated Proteins
Ion Selectivity and Conductance
Voltage Dependence
Gating Inhibitors
Pore Blockers
Tissue Distribution
Functional Assays
Physiological Functions
General Comments
References
Gene and Protein Information
Species TM P Loops AA Chromosomal Location Gene Symbol Gene Name Reference
Human 6 1 911 8q13.2 KCNB2 potassium voltage-gated channel, Shab-related subfamily, member 2 18
Mouse 6 1 907 1 A3 Kcnb2 potassium voltage gated channel, Shab-related subfamily, member 2
Rat 6 1 907 5q11 Kcnb2 potassium voltage gated channel, Shab-related subfamily, member 2
Previous and Unofficial Names
CDRK
9630047L19Rik
BB130875
Kv2.2
potassium voltage gated channel, Shab-related subfamily, member 2
potassium voltage-gated channel subfamily B member 2
voltage-gated potassium channel subunit Kv2.2
Database Links
Ensembl ENSG00000182674 (Hs), ENSMUSG00000030525 (Mm), ENSRNOG00000028991 (Rn)
Entrez Gene 9312 (Hs), 98741 (Mm), 117105 (Rn)
GeneCards KCNB2 (Hs)
GenitoUrinary Development Molecular Anatomy Project Kcnb2 (Mm)
HomoloGene 31263 (Hs)
Human Protein Reference Database 09666 (Hs)
InterPro Q92953 (Hs), A6H8H5 (Mm), Q63099 (Rn)
KEGG Gene hsa:9312 (Hs), mmu:98741 (Mm), rno:117105 (Rn)
OMIM 607738 (Hs)
PharmGKB Gene PA30025 (Hs)
PhosphoSitePlus Q92953 (Hs), A6H8H5 (Mm), Q63099 (Rn)
Protein Ontology (PRO) PRO:000000723 (Hs)
RefSeq Nucleotide NM_004770 (Hs), NM_001098528 (Mm), NM_054000 (Rn)
RefSeq Protein NP_004761 (Hs), NP_001091998 (Mm), NP_446452 (Rn)
TreeFam ENSG00000182674 (Hs), ENSMUSG00000030525 (Mm), ENSRNOG00000028991 (Rn)
UniGene Hs. 661102 (Hs)
UniProt Q92953 (Hs), A6H8H5 (Mm), Q63099 (Rn)
Wikipedia Kv2.2
Potassium channels
Search for 3D structures on the PDB
Search by keyword: Voltage-gated potassium channels Kv2.2 Search by accession number: Q63099, A6H8H5, Q92953
Associated Proteins
Heteromeric Pore-forming Subunits
Name References
Kvβ4 5
Kv9 17
Kv8.1 16
Auxiliary Subunits
Name References
Not determined
Other Associated Proteins
Name References
KChaP 19
Ion Selectivity and Conductance
Species:  Dog
Rank order:  K+ [15.0 pS]
References:  18
Voltage Dependence
  V0.5 (mV)  τ (msec)  Reference  Cell type  Species 
Activation  - 97.0 18 Xenopus laevis oocyte Dog
Inactivation  - -
Comments  A slowly activating K+ current, non-inactivating
Associated subunits (Human)
Kv5.1, Kv6.1-6.4, Kv8.1-8.2 and Kv9.1-9.3
Gating inhibitors
Key to terms and symbols Click column headers to sort
Ligand Sp. Affinity Units Concentration range (M) Holding voltage (mV) Reference
ScTx1 Rn 7.6 pIC50 - - 4
Pore Blockers
Key to terms and symbols View all chemical structures Click column headers to sort
Ligand Sp. Affinity Units Concentration range (M) Holding voltage (mV) Reference
quinine Hs 4.9 pIC50 - - 18
4-aminopyridine Hs 2.8 pIC50 - - 18
tetraethylammonium Hs 2.6 pIC50 - - 18
Pore Blocker Comments
Phencyclidine (PCP) also inhbits human Kv2.2 from human colonic circular smooth muscle (pIC50 roughly 4.3) [6]
Tissue Distribution
Gastric cancer cells
Species:  Human
Technique:  RT-PCR
References:  11
Pancreatic islet delta cells
Species:  Human
Technique:  Immunohistochemistry
References:  21
Gastrointestinal smooth muscle, mesenteric artery smooth muscle
Species:  Rat
Technique:  RT-PCR
References:  20
Brain (olfactory bulb [granule cell layer > olfactory tubercle] > cortex > hippocampus > cerebellum), hypothalam, ventricle, tongue, sympathetic neurons
Species:  Rat
Technique:  Immunocytochemistry
References:  3,7-10,12-13
Tissue Distribution Comments
Also; Rabbit myocytes from corpus cavernosum (RT-PCR, [15]), Ferret cardiac myocytes (In situ hybridisation, [2]);
Functional Assays
Gene cloning and voltage clamp
Species:  Human
Tissue:  Xenopus laevis oocytes
Response measured:  slowly activating K+ current
References:  18
Physiological Functions
Maintaining membrane potential, modulating electrical excitability in neurons
Species:  Rat
Tissue:  Superior cervical ganglion (SCG)
References:  14
Maintaining membrane potential, modulating electrical excitability in neurons
Species:  None
Tissue:  Xenopus laevis
References:  1
Physiological Functions Comments
Species ([1]): Xenopus laevis embryonic spinal neurons
Physiological Consequences of Altering Gene Expression
Not Determined
Clinically-Relevant Mutations and Pathophysiology
Not Determined
Gene Expression and Pathophysiology
Not Determined
Biologically Significant Variants
Not Determined
General Comments
The angiotensin II type 1 receptor mediates inhibition of Kv2.2 in the brainstem and hypothalamic neurons. It is a member of the mammalian Shab-related family.

REFERENCES

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1. Blaine, JT; Taylor, AD; Ribera, AB. (2004) Carboxyl tail region of the Kv2.2 subunit mediates novel developmental regulation of channel density. J. Neurophysiol., 92 (6): 3446-54. [PMID:15306626]

2. Brahmajothi, MV; Morales, MJ; Liu, S; Rasmusson, RL; Campbell, DL; Strauss, HC. (1996) In situ hybridization reveals extensive diversity of K+ channel mRNA in isolated ferret cardiac myocytes. Circ. Res., 78 (6): 1083-9. [PMID:8635239]

3. Dixon, JE; McKinnon, D. (1996) Potassium channel mRNA expression in prevertebral and paravertebral sympathetic neurons. Eur. J. Neurosci., 8 (1): 183-91. [PMID:8713462]

4. Escoubas, P; Diochot, S; Célérier, ML; Nakajima, T; Lazdunski, M. (2002) Novel tarantula toxins for subtypes of voltage-dependent potassium channels in the Kv2 and Kv4 subfamilies. Mol. Pharmacol., 62 (1): 48-57. [PMID:12065754]

5. Fink, M; Duprat, F; Lesage, F; Heurteaux, C; Romey, G; Barhanin, J; Lazdunski, M. (1996) A new K+ channel beta subunit to specifically enhance Kv2.2 (CDRK) expression. J. Biol. Chem., 271 (42): 26341-8. [PMID:8824288]

6. Frey, BW; Lynch, FT; Kinsella, JM; Horowitz, B; Sanders, KM; Carl, A. (2000) Blocking of cloned and native delayed rectifier K channels from visceral smooth muscles by phencyclidine. Neurogastroenterol. Motil., 12 (6): 509-16. [PMID:11123705]

7. Gelband, CH; Warth, JD; Mason, HS; Zhu, M; Moore, JM; Kenyon, JL; Horowitz, B; Sumners, C. (1999) Angiotensin II type 1 receptor-mediated inhibition of K+ channel subunit kv2.2 in brain stem and hypothalamic neurons. Circ. Res., 84 (3): 352-9. [PMID:10024310]

8. Hwang, PM; Cunningham, AM; Peng, YW; Snyder, SH. (1993) CDRK and DRK1 K+ channels have contrasting localizations in sensory systems. Neuroscience, 55 (3): 613-20. [PMID:8413924]

9. Hwang, PM; Fotuhi, M; Bredt, DS; Cunningham, AM; Snyder, SH. (1993) Contrasting immunohistochemical localizations in rat brain of two novel K+ channels of the Shab subfamily. J. Neurosci., 13 (4): 1569-76. [PMID:8463836]

10. Hwang, PM; Glatt, CE; Bredt, DS; Yellen, G; Snyder, SH. (1992) A novel K+ channel with unique localizations in mammalian brain: molecular cloning and characterization. Neuron, 8 (3): 473-81. [PMID:1550672]

11. Lan, M; Shi, Y; Han, Z; Hao, Z; Pan, Y; Liu, N; Guo, C; Hong, L; Wang, J; Qiao, T; Fan, D. (2005) Expression of delayed rectifier potassium channels and their possible roles in proliferation of human gastric cancer cells. Cancer Biol. Ther., 4 (12): 1342-7. [PMID:16258262]

12. Lim, ST; Antonucci, DE; Scannevin, RH; Trimmer, JS. (2000) A novel targeting signal for proximal clustering of the Kv2.1 K+ channel in hippocampal neurons. Neuron, 25 (2): 385-97. [PMID:10719893]

13. Maletic-Savatic, M; Lenn, NJ; Trimmer, JS. (1995) Differential spatiotemporal expression of K+ channel polypeptides in rat hippocampal neurons developing in situ and in vitro. J. Neurosci., 15 (5 Pt 2): 3840-51. [PMID:7751950]

14. Malin, SA; Nerbonne, JM. (2002) Delayed rectifier K+ currents, IK, are encoded by Kv2 alpha-subunits and regulate tonic firing in mammalian sympathetic neurons. J. Neurosci., 22 (23): 10094-105. [PMID:12451110]

15. Malysz, J; Farrugia, G; Ou, Y; Szurszewski, JH; Nehra, A; Gibbons, SJ. (2002) The Kv2.2 alpha subunit contributes to delayed rectifier K(+) currents in myocytes from rabbit corpus cavernosum. J. Androl., 23 (6): 899-910. [PMID:12399537]

16. Salinas, M; de Weille, J; Guillemare, E; Lazdunski, M; Hugnot, JP. (1997) Modes of regulation of shab K+ channel activity by the Kv8.1 subunit. J. Biol. Chem., 272 (13): 8774-80. [PMID:9079713]

17. Salinas, M; Duprat, F; Heurteaux, C; Hugnot, JP; Lazdunski, M. (1997) New modulatory alpha subunits for mammalian Shab K+ channels. J. Biol. Chem., 272 (39): 24371-9. [PMID:9305895]

18. Schmalz, F; Kinsella, J; Koh, SD; Vogalis, F; Schneider, A; Flynn, ER; Kenyon, JL; Horowitz, B. (1998) Molecular identification of a component of delayed rectifier current in gastrointestinal smooth muscles. Am. J. Physiol., 274 (5 Pt 1): G901-11. [PMID:9612272]

19. Wible, BA; Yang, Q; Kuryshev, YA; Accili, EA; Brown, AM. (1998) Cloning and expression of a novel K+ channel regulatory protein, KChAP. J. Biol. Chem., 273 (19): 11745-51. [PMID:9565597]

20. Xu, C; Lu, Y; Tang, G; Wang, R. (1999) Expression of voltage-dependent K(+) channel genes in mesenteric artery smooth muscle cells. Am. J. Physiol., 277 (5 Pt 1): G1055-63. [PMID:10564112]

21. Yan, L; Figueroa, DJ; Austin, CP; Liu, Y; Bugianesi, RM; Slaughter, RS; Kaczorowski, GJ; Kohler, MG. (2004) Expression of voltage-gated potassium channels in human and rhesus pancreatic islets. Diabetes, 53 (3): 597-607. [PMID:14988243]

To cite this database page, please use the following:

K. George Chandy, Stephan Grissmer, George A. Gutman, Michel Lazdunski, David Mckinnon, Luis A. Pardo, Gail A. Robertson, Bernardo Rudy, Michael C. Sanguinetti, Walter Stühmer, Xiaoliang Wang.
Voltage-gated potassium channels: Kv2.2. Last modified on 01/07/2010. Accessed on 26/05/2013. IUPHAR database (IUPHAR-DB), http://iuphar-db.org/DATABASE/ObjectDisplayForward?objectId=547.


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