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TRPV5

Family: Transient Receptor Potential channels

Contents:
Gene and Protein Information
Previous and Unofficial Names
Database Links
Associated Proteins
Activators
Tissue Distribution
Functional Assays
Physiological Functions
Physiological Consequences of Altering Gene Expression
Phenotypes, Alleles and Disease Models
References
Gene and Protein Information
Species TM P Loops AA Chromosomal Location Gene Symbol Gene Name Reference
Human 6 1 729 7q35 TRPV5 transient receptor potential cation channel, subfamily V, member 5 6
Mouse 6 1 723 6 B2.1 Trpv5 transient receptor potential cation channel, subfamily V, member 5 17
Rat 6 1 723 4q23 Trpv5 transient receptor potential cation channel, subfamily V, member 5 7-8
Previous and Unofficial Names
OTRPC3
ECAC1
CaT2
ECaC
ECaC1
epithelial calcium channel 1
calcium transporter 2
osm-9-like TRP channel 3
transient receptor potential cation channel subfamily V member 5
transient receptor potential cation channel, subfamily V, member 5
Database Links
Ensembl ENSG00000127412 (Hs), ENSMUSG00000036899 (Mm), ENSRNOG00000015394 (Rn)
Entrez Gene 56302 (Hs), 194352 (Mm), 116469 (Rn)
GeneCards TRPV5 (Hs)
GenitoUrinary Development Molecular Anatomy Project Trpv5 (Mm)
HomoloGene 10520 (Hs)
Human Protein Reference Database 05981 (Hs)
InterPro Q9NQA5 (Hs), P69744 (Mm), Q9JIP0 (Rn)
KEGG Gene hsa:56302 (Hs), mmu:194352 (Mm), rno:116469 (Rn)
OMIM 606679 (Hs)
PharmGKB Gene PA35045 (Hs)
PhosphoSitePlus Q9NQA5 (Hs), P69744 (Mm)
RefSeq Nucleotide NM_019841 (Hs), NM_001007572 (Mm), NM_053787 (Rn)
RefSeq Protein NP_062815 (Hs), NP_001007573 (Mm), NP_446239 (Rn)
TreeFam ENSG00000127412 (Hs), ENSMUSG00000036899 (Mm), ENSRNOG00000015394 (Rn)
UniGene Hs. 283369 (Hs)
UniProt Q9NQA5 (Hs), P69744 (Mm), Q9JIP0 (Rn)
Wikipedia TRPV5
Transient Receptor Potential channels
Search for 3D structures on the PDB
Search by keyword: Transient Receptor Potential channels TRPV5 Search by accession number: P69744, Q9NQA5, Q9JIP0
Associated Proteins
Heteromeric Pore-forming Subunits
Name References
TRPV6 3
Auxiliary Subunits
Name References
Not determined
Other Associated Proteins
Name References
calmodulin 5
Rab11a 10
BSPRY 13
NHERF4 12
80K-H 1
S100A10/annexin 2 11
Activators (Human)
constitutively active (with strong buffering of intracellular Ca2+)
Other channel blockers (Human)
Pb2+ = Cu2+ = Gd3+ > Cd2+ > Zn2+ > La3+ > Co2+ > Fe2+
Activator Comments
TRPV5 is constitutively active [15]. Its function is modulated by protons [16,18-19].
Tissue Distribution
Kidney, prostate, testes, placenta, pancreas, brain.
Species:  Human
Technique:  RT-PCR
References:  6
Kidney.
Species:  Mouse
Technique:  RT-PCR
References:  17
Osteoclasts.
Species:  Mouse
Technique:  RT-PCR and immunocytochemistry.
References:  14
Kidney.
Species:  Rat
Technique:  Northern Blot and RT-PCR.
References:  7
Functional Assays
Patch clamp (whole-cell recordings).
Species:  Rat
Tissue:  Kidney.
Response measured:  Ca2+-evoked currents.
References:  8
Two-electrode voltage clamp.
Species:  Rat
Tissue:  Xenopus laevis oocytes injected with TRPV5 cDNA.
Response measured:  Ca2+-evoked currents.
References:  7
Physiological Functions
Ca2+ (re)absorption.
Species:  Mouse
Tissue:  Kidney, intestines, bone.
References:  14
Ca2+ (re)absorption.
Species:  Human
Tissue:  Kidney, intestines, bone.
References:  6
Physiological Consequences of Altering Gene Expression
Osteporosis
Species:  Mouse
Tissue:  Bone
Technique:  TRPV5 knockout
References:  2
Diabetes mellitus type 2
Species:  Rat
Tissue:  Pancreatic islet cells
Technique:  Downregulation of TRPV5
References:  4
Hypercalciuria
Species:  Mouse
Tissue:  Kidney
Technique:  Downregulation/knockout mouse
References:  2,9
Phenotypes, Alleles and Disease Models Mouse data from MGI

Click here to show/hide data

Allele Composition & genetic background Accession Phenotype Id Phenotype Reference
Trpv5tm1Bind Trpv5tm1Bind/Trpv5tm1Bind
involves: 129 * C57BL/6		 MGI:2429764  MP:0003795 abnormal bone structure PMID: 14679186 
Trpv5tm1Bind Trpv5tm1Bind/Trpv5tm1Bind
involves: 129 * C57BL/6		 MGI:2429764  MP:0004231 abnormal calcium ion homeostasis PMID: 14679186 
Trpv5tm1Bind Trpv5tm1Bind/Trpv5tm1Bind
involves: 129 * C57BL/6		 MGI:2429764  MP:0004214 abnormal long bone diaphysis morphology PMID: 14679186 
Trpv5tm1Bind Trpv5tm1Bind/Trpv5tm1Bind
involves: 129 * C57BL/6		 MGI:2429764  MP:0010111 abnormal renal calcium reabsorption PMID: 14679186 
Trpv5tm1Bind Trpv5tm1Bind/Trpv5tm1Bind
involves: 129 * C57BL/6		 MGI:2429764  MP:0005400 abnormal vitamin level PMID: 14679186 
Trpv5tm1Bind Trpv5tm1Bind/Trpv5tm1Bind
involves: 129 * C57BL/6		 MGI:2429764  MP:0009346 decreased cancellous bone thickness PMID: 14679186 
Trpv5tm1Bind Trpv5tm1Bind/Trpv5tm1Bind
involves: 129 * C57BL/6		 MGI:2429764  MP:0000135 decreased cortical bone thickness PMID: 14679186 
Trpv5tm1Bind Trpv5tm1Bind/Trpv5tm1Bind
involves: 129 * C57BL/6		 MGI:2429764  MP:0009350 decreased urine pH PMID: 14679186 
Trpv5tm1Bind Trpv5tm1Bind/Trpv5tm1Bind
involves: 129 * C57BL/6		 MGI:2429764  MP:0005441 hypercalciuria PMID: 14679186 
Trpv5tm1Bind Trpv5tm1Bind/Trpv5tm1Bind
involves: 129 * C57BL/6		 MGI:2429764  MP:0002901 hyperphosphaturia PMID: 14679186 
Trpv5tm1Bind Trpv5tm1Bind/Trpv5tm1Bind
involves: 129 * C57BL/6		 MGI:2429764  MP:0004984 increased osteoclast cell number PMID: 14679186 
Trpv5tm1Bind Trpv5tm1Bind/Trpv5tm1Bind
involves: 129 * C57BL/6		 MGI:2429764  MP:0001762 polyuria PMID: 14679186 

REFERENCES

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1. Gkika, D; Hsu, YJ; van der Kemp, AW; Christakos, S; Bindels, RJ; Hoenderop, JG. (2006) Critical role of the epithelial Ca2+ channel TRPV5 in active Ca2+ reabsorption as revealed by TRPV5/calbindin-D28K knockout mice. J. Am. Soc. Nephrol., 17 (11): 3020-7. [PMID:17005931]

2. Hoenderop, JG; van Leeuwen, JP; van der Eerden, BC; Kersten, FF; van der Kemp, AW; Mérillat, AM; Waarsing, JH; Rossier, BC; Vallon, V; Hummler, E; Bindels, RJ. (2003) Renal Ca2+ wasting, hyperabsorption, and reduced bone thickness in mice lacking TRPV5. J. Clin. Invest., 112 (12): 1906-14. [PMID:14679186]

3. Hoenderop, JG; Voets, T; Hoefs, S; Weidema, F; Prenen, J; Nilius, B; Bindels, RJ. (2003) Homo- and heterotetrameric architecture of the epithelial Ca2+ channels TRPV5 and TRPV6. EMBO J., 22 (4): 776-85. [PMID:12574114]

4. Janssen, SW; Hoenderop, JG; Hermus, AR; Sweep, FC; Martens, GJ; Bindels, RJ. (2002) Expression of the novel epithelial Ca2+ channel ECaC1 in rat pancreatic islets. J. Histochem. Cytochem., 50 (6): 789-98. [PMID:12019295]

5. Lambers, TT; Weidema, AF; Nilius, B; Hoenderop, JG; Bindels, RJ. (2004) Regulation of the mouse epithelial Ca2(+) channel TRPV6 by the Ca(2+)-sensor calmodulin. J. Biol. Chem., 279 (28): 28855-61. [PMID:15123711]

6. Müller, D; Hoenderop, JG; Meij, IC; van den Heuvel, LP; Knoers, NV; den Hollander, AI; Eggert, P; Garcia-Nieto, V; Claverie-Martín, F; Bindels, RJ. (2000) Molecular cloning, tissue distribution, and chromosomal mapping of the human epithelial Ca2+ channel (ECAC1). Genomics, 67 (1): 48-53. [PMID:10945469]

7. Peng, JB; Chen, XZ; Berger, UV; Vassilev, PM; Brown, EM; Hediger, MA. (2000) A rat kidney-specific calcium transporter in the distal nephron. J. Biol. Chem., 275 (36): 28186-94. [PMID:10875938]

8. Suzuki, M; Ishibashi, K; Ooki, G; Tsuruoka, S; Imai, M. (2000) Electrophysiologic characteristics of the Ca-permeable channels, ECaC and CaT, in the kidney. Biochem. Biophys. Res. Commun., 274 (2): 344-9. [PMID:10913341]

9. van Abel, M; Hoenderop, JG; van der Kemp, AW; Friedlaender, MM; van Leeuwen, JP; Bindels, RJ. (2005) Coordinated control of renal Ca(2+) transport proteins by parathyroid hormone. Kidney Int., 68 (4): 1708-21. [PMID:16164647]

10. van de Graaf, SF; Chang, Q; Mensenkamp, AR; Hoenderop, JG; Bindels, RJ. (2006) Direct interaction with Rab11a targets the epithelial Ca2+ channels TRPV5 and TRPV6 to the plasma membrane. Mol. Cell. Biol., 26 (1): 303-12. [PMID:16354700]

11. van de Graaf, SF; Hoenderop, JG; Gkika, D; Lamers, D; Prenen, J; Rescher, U; Gerke, V; Staub, O; Nilius, B; Bindels, RJ. (2003) Functional expression of the epithelial Ca(2+) channels (TRPV5 and TRPV6) requires association of the S100A10-annexin 2 complex. EMBO J., 22 (7): 1478-87. [PMID:12660155]

12. van de Graaf, SF; Hoenderop, JG; van der Kemp, AW; Gisler, SM; Bindels, RJ. (2006) Interaction of the epithelial Ca2+ channels TRPV5 and TRPV6 with the intestine- and kidney-enriched PDZ protein NHERF4. Pflugers Arch., 452 (4): 407-17. [PMID:16565876]

13. van de Graaf, SF; van der Kemp, AW; van den Berg, D; van Oorschot, M; Hoenderop, JG; Bindels, RJ. (2006) Identification of BSPRY as a novel auxiliary protein inhibiting TRPV5 activity. J. Am. Soc. Nephrol., 17 (1): 26-30. [PMID:16380433]

14. van der Eerden, BC; Hoenderop, JG; de Vries, TJ; Schoenmaker, T; Buurman, CJ; Uitterlinden, AG; Pols, HA; Bindels, RJ; van Leeuwen, JP. (2005) The epithelial Ca2+ channel TRPV5 is essential for proper osteoclastic bone resorption. Proc. Natl. Acad. Sci. U.S.A., 102 (48): 17507-12. [PMID:16291808]

15. Vennekens, R; Hoenderop, JG; Prenen, J; Stuiver, M; Willems, PH; Droogmans, G; Nilius, B; Bindels, RJ. (2000) Permeation and gating properties of the novel epithelial Ca(2+) channel. J. Biol. Chem., 275 (6): 3963-9. [PMID:10660551]

16. Vennekens, R; Prenen, J; Hoenderop, JG; Bindels, RJ; Droogmans, G; Nilius, B. (2001) Modulation of the epithelial Ca2+ channel ECaC by extracellular pH. Pflugers Arch., 442 (2): 237-42. [PMID:11417220]

17. Weber, K; Erben, RG; Rump, A; Adamski, J. (2001) Gene structure and regulation of the murine epithelial calcium channels ECaC1 and 2. Biochem. Biophys. Res. Commun., 289 (5): 1287-94. [PMID:11741335]

18. Yeh, BI; Kim, YK; Jabbar, W; Huang, CL. (2005) Conformational changes of pore helix coupled to gating of TRPV5 by protons. EMBO J., 24 (18): 3224-34. [PMID:16121193]

19. Yeh, BI; Sun, TJ; Lee, JZ; Chen, HH; Huang, CL. (2003) Mechanism and molecular determinant for regulation of rabbit transient receptor potential type 5 (TRPV5) channel by extracellular pH. J. Biol. Chem., 278 (51): 51044-52. [PMID:14525991]

To cite this database page, please use the following:

David E. Clapham, Bernd Nilius, Grzegorz Owsianik.
Transient Receptor Potential channels: TRPV5. Last modified on 30/11/2011. Accessed on 25/05/2013. IUPHAR database (IUPHAR-DB), http://iuphar-db.org/DATABASE/ObjectDisplayForward?objectId=511.


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