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TRPV3

Family: Transient Receptor Potential channels

Contents:
Gene and Protein Information
Previous and Unofficial Names
Database Links
Associated Proteins
Ion Selectivity and Conductance
Voltage Dependence
Activators
Pore Blockers
Tissue Distribution
Functional Assays
Physiological Functions
Phenotypes, Alleles and Disease Models
Clinically-Relevant Mutations and Pathophysiology
References
Gene and Protein Information
Species TM P Loops AA Chromosomal Location Gene Symbol Gene Name Reference
Human 6 1 790 17p13.3 TRPV3 transient receptor potential cation channel, subfamily V, member 3 11
Mouse 6 1 791 11 B4 Trpv3 transient receptor potential cation channel, subfamily V, member 3 7
Rat 6 1 791 10q24 Trpv3 transient receptor potential cation channel, subfamily V, member 3
Previous and Unofficial Names
VRL-3
VRL3
heat sensitive channel TRPV3
transient receptor potential cation channel subfamily V member 3
transient receptor potential cation channel, subfamily V, member 3
Nh
1110036I10Rik
AI644701
Database Links
DrugBank Target 5838 (Hs)
Ensembl ENSG00000167723 (Hs), ENSMUSG00000043029 (Mm), ENSRNOG00000019606 (Rn)
Entrez Gene 162514 (Hs), 246788 (Mm), 497948 (Rn)
GeneCards TRPV3 (Hs)
GenitoUrinary Development Molecular Anatomy Project Trpv3 (Mm)
HomoloGene 17040 (Hs)
Human Protein Reference Database 06141 (Hs)
InterPro Q8NET8 (Hs), Q8K424 (Mm)
KEGG Gene hsa:162514 (Hs), mmu:246788 (Mm), rno:497948 (Rn)
OMIM 162514 (Hs)
PharmGKB Gene PA38481 (Hs)
PhosphoSitePlus Q8NET8 (Hs), Q8K424 (Mm)
Protein Ontology (PRO) PRO:000001060 (Hs)
RefSeq Nucleotide NM_145068 (Hs), NM_145099 (Mm), NM_001025757 (Rn)
RefSeq Protein NP_659505 (Hs), NP_659567 (Mm), NP_001020928 (Rn)
TreeFam ENSG00000167723 (Hs), ENSMUSG00000043029 (Mm), ENSRNOG00000019606 (Rn)
UniGene Hs. 446255 (Hs)
UniProt Q8NET8 (Hs), Q8K424 (Mm)
Wikipedia TRPV3
Transient Receptor Potential channels
Search for 3D structures on the PDB
Search by keyword: Transient Receptor Potential channels TRPV3 Search by accession number: Q8NET8, Q8K424
Associated Proteins
Heteromeric Pore-forming Subunits
Name References
TRPV1 9
Auxiliary Subunits
Name References
Not determined
Other Associated Proteins
Name References
Not determined
Ion Selectivity and Conductance
Species:  Human
Rank order:  Ca2+ > Cs+ [172.0 pS] = K+ = Na+
References:  11
Voltage Dependence
  V0.5 (mV)  τ (msec)  Reference  Cell type  Species 
Activation  38.7 – 80.7 - 2 HEK 293 cells transfected with the TRPV3 vector. Mouse
Inactivation  - -
Comments  Activation by two identical consecutive heat stimuli shifts V0.5 to the less positive potential without affecting slope factor (31.7 ± 1.3 vs 36.6 ± 1.9 mV, respectively).
Other chemical activators (Human)
NO-mediated cysteine S-nitrosylation
Physical activators (Human)
depolarization (V½ ~ +80 mV, reduced to more negative values following heat stimuli), heat (23°C - 39°C, temperature threshold reduces with repeated heat challenge)
Activators
Key to terms and symbols View all chemical structures Click column headers to sort
Ligand Sp. Affinity Units Concentration range (M) Holding voltage (mV) Reference
eugenol Mm - - 3x10-3 -60.0 10
camphor Mm - - 2x10-3 -60.0 6
cinnamaldehyde Mm - - 1x10-3 -80.0 – 80.0 5
carvacrol Mm - - 5x10-4 -60.0 10
thymol Mm - - 5x10-4 -60.0 10
2-APB Mm 4.6 pEC50 - -80.0 – 80.0 4
diphenylboronic anhydride Mm 4.1 – 4.2 pEC50 - -80.0 – 80.0 2
menthol Mm 1.7 pEC50 - -80.0 – 80.0 5
Activator Comments
Sequentially applied high concentrations of diphenylboronic anhydride inhibit TRPV3.
Chemical or thermal activation of TRPV3 evokes biphasic currents [2].
TRPV3 is also activated by innocuous heat [7-8,11].
Pore Blockers
Key to terms and symbols View all chemical structures Click column headers to sort
Ligand Sp. Affinity Units Concentration range (M) Holding voltage (mV) Reference
ruthenium red Mm - - 1x10-6 -60.0 7
diphenyltetrahydrofuran Mm 5.0 – 5.2 pIC50 - -80.0 – 80.0 2
Pore Blocker Comments
Value for diphenyltetrahydrofuran represents action on initial current component [2].

Explore drug-target interactions for this set of compounds using iPHACE

Tissue Distribution
Dorsal root ganglia, brain, spinal cord, skin, testes, stomach, tongue, trachea, small intestine, placenta, hair follicles.
Species:  Human
Technique:  In situ hybridisation, RT-PCR, Northern Blot.
References:  11
Dorsal root ganglia, brain, spinal cord, stomach, intestine, adipose tissue, pituitary, placenta.
Species:  Human
Technique:  RT-PCR
References:  9
Skin keratinocytes, hair follicles.
Species:  Mouse
Technique:  In situ hybridisation, Northern Blot.
References:  7
Functional Assays
Patch clamp (whole-cell recordings).
Species:  Mouse
Tissue:  CHO cells transfected with the TRPV3 vector, mouse keratinocytes.
Response measured:  Activation by innocuous heat.
References:  7
Patch clamp (whole-cell and single channel recordings), intracellular Ca2+ imaging.
Species:  Human
Tissue:  CHO-K1 cells transfected with the TRPV3 vector.
Response measured:  Activation by innocuous heat.
References:  11
Patch clamp (whole-cell recordings), intracellular Ca2+ imaging.
Species:  Mouse
Tissue:  Mouse 308 keratinocytes.
Response measured:  Activation by innocuous heat.
References:  3
Physiological Functions
Thermosensation (innocuous heat).
Species:  Mouse
Tissue:  Keratinocytes and sensory neurones.
References:  7
Detection and integration of innocuous and noxious stimuli (acute nociceptive pain).
Species:  Mouse
Tissue:  Sensory neurones.
References:  6
Thermosensation (innocuous heat).
Species:  Human
Tissue:  Sensory neurones.
References:  9,11
Phenotypes, Alleles and Disease Models Mouse data from MGI

Click here to show/hide data

Allele Composition & genetic background Accession Phenotype Id Phenotype Reference
Trpv3tm1Apat Trpv3tm1Apat/Trpv3tm1Apat
involves: 129S1/Sv * 129X1/SvJ * C57BL/6J		 MGI:2181407  MP:0000367 abnormal coat/ hair morphology PMID: 15746429 
Tg(KRT14-cre)1Amc|Trpv3tm1.1Clph Tg(KRT14-cre)1Amc/0,Trpv3tm1.1Clph/Trpv3tm1.1Clph
involves: 129S4/SvJae * 129S4/SvJaeSor * C57BL/6 * C57BL/6J * CBA * Swiss Webster		 MGI:2181407  MGI:2450263  MP:0009594 abnormal corneocyte envelope morphology PMID: 20403327 
Trpv3tm1.2Clph Trpv3tm1.2Clph/Trpv3tm1.2Clph
involves: 129S4/SvJae * 129S4/SvJaeSor * C57BL/6 * C57BL/6J * CBA		 MGI:2181407  MP:0009594 abnormal corneocyte envelope morphology PMID: 20403327 
Tg(KRT14-cre)1Amc|Trpv3tm1.1Clph Tg(KRT14-cre)1Amc/0,Trpv3tm1.1Clph/Trpv3tm1.1Clph
involves: 129S4/SvJae * 129S4/SvJaeSor * C57BL/6 * C57BL/6J * CBA * Swiss Webster		 MGI:2181407  MGI:2450263  MP:0001240 abnormal epidermis stratum corneum morphology PMID: 20403327 
Trpv3tm1.2Clph Trpv3tm1.2Clph/Trpv3tm1.2Clph
involves: 129S4/SvJae * 129S4/SvJaeSor * C57BL/6 * C57BL/6J * CBA		 MGI:2181407  MP:0001240 abnormal epidermis stratum corneum morphology PMID: 20403327 
Tg(KRT14-cre)1Amc|Trpv3tm1.1Clph Tg(KRT14-cre)1Amc/0,Trpv3tm1.1Clph/Trpv3tm1.1Clph
involves: 129S4/SvJae * 129S4/SvJaeSor * C57BL/6 * C57BL/6J * CBA * Swiss Webster		 MGI:2181407  MGI:2450263  MP:0000383 abnormal hair follicle orientation PMID: 20403327 
Trpv3tm1.2Clph Trpv3tm1.2Clph/Trpv3tm1.2Clph
involves: 129S4/SvJae * 129S4/SvJaeSor * C57BL/6 * C57BL/6J * CBA		 MGI:2181407  MP:0000383 abnormal hair follicle orientation PMID: 20403327 
Trpv3+|Trpv3Nh Trpv3Nh/Trpv3+
DS		 MGI:2181407  MP:0002073 abnormal hair growth PMID: 9250484 
Trpv3tm1Apat Trpv3tm1Apat/Trpv3tm1Apat
involves: 129S1/Sv * 129X1/SvJ * C57BL/6J		 MGI:2181407  MP:0003453 abnormal keratinocyte physiology PMID: 15746429 
Trpv3tm1.2Clph Trpv3tm1.2Clph/Trpv3tm1.2Clph
involves: 129S4/SvJae * 129S4/SvJaeSor * C57BL/6 * C57BL/6J * CBA		 MGI:2181407  MP:0003453 abnormal keratinocyte physiology PMID: 20403327 
Trpv3tm1Apat Trpv3tm1Apat/Trpv3tm1Apat
involves: 129S1/Sv * 129X1/SvJ * C57BL/6J		 MGI:2181407  MP:0003663 abnormal thermosensation PMID: 15746429 
Tg(KRT14-cre)1Amc|Trpv3tm1.1Clph Tg(KRT14-cre)1Amc/0,Trpv3tm1.1Clph/Trpv3tm1.1Clph
involves: 129S4/SvJae * 129S4/SvJaeSor * C57BL/6 * C57BL/6J * CBA * Swiss Webster		 MGI:2181407  MGI:2450263  MP:0001274 curly vibrissae PMID: 20403327 
Trpv3tm1.2Clph Trpv3tm1.2Clph/Trpv3tm1.2Clph
B6.129S4-Trpv3		 MGI:2181407  MP:0001274 curly vibrissae PMID: 20403327 
Trpv3tm1.2Clph Trpv3tm1.2Clph/Trpv3tm1.2Clph
involves: 129S4/SvJae * 129S4/SvJaeSor * C57BL/6 * C57BL/6J * CBA		 MGI:2181407  MP:0001274 curly vibrissae PMID: 20403327 
Trpv3+|Trpv3Nh Trpv3Nh/Trpv3+
DS		 MGI:2181407  MP:0001194 dermatitis PMID: 9250484 
Tg(KRT14-cre)1Amc|Trpv3tm1.1Clph Tg(KRT14-cre)1Amc/0,Trpv3tm1.1Clph/Trpv3tm1.1Clph
involves: 129S4/SvJae * 129S4/SvJaeSor * C57BL/6 * C57BL/6J * CBA * Swiss Webster		 MGI:2181407  MGI:2450263  MP:0003853 dry skin PMID: 20403327 
Trpv3tm1.2Clph Trpv3tm1.2Clph/Trpv3tm1.2Clph
involves: 129S4/SvJae * 129S4/SvJaeSor * C57BL/6 * C57BL/6J * CBA		 MGI:2181407  MP:0003853 dry skin PMID: 20403327 
Tg(KRT14-cre)1Amc|Trpv3tm1.1Clph Tg(KRT14-cre)1Amc/0,Trpv3tm1.1Clph/Trpv3tm1.1Clph
involves: 129S4/SvJae * 129S4/SvJaeSor * C57BL/6 * C57BL/6J * CBA * Swiss Webster		 MGI:2181407  MGI:2450263  MP:0003454 erythroderma PMID: 20403327 
Trpv3tm1.2Clph Trpv3tm1.2Clph/Trpv3tm1.2Clph
involves: 129S4/SvJae * 129S4/SvJaeSor * C57BL/6 * C57BL/6J * CBA		 MGI:2181407  MP:0003454 erythroderma PMID: 20403327 
Trpv3+|Trpv3Nh Trpv3Nh/Trpv3+
DS		 MGI:2181407  MP:0002412 increased susceptibility to bacterial infection PMID: 9250484 
Trpv3tm1Apat Trpv3tm1Apat/Trpv3tm1Apat
involves: 129S1/Sv * 129X1/SvJ * C57BL/6J		 MGI:2181407  MP:0001973 increased thermal nociceptive threshold PMID: 15746429 
Tg(KRT14-cre)1Amc|Trpv3tm1.1Clph Tg(KRT14-cre)1Amc/0,Trpv3tm1.1Clph/Trpv3tm1.1Clph
involves: 129S4/SvJae * 129S4/SvJaeSor * C57BL/6 * C57BL/6J * CBA * Swiss Webster		 MGI:2181407  MGI:2450263  MP:0001192 scaly skin PMID: 20403327 
Trpv3tm1.2Clph Trpv3tm1.2Clph/Trpv3tm1.2Clph
involves: 129S4/SvJae * 129S4/SvJaeSor * C57BL/6 * C57BL/6J * CBA		 MGI:2181407  MP:0001192 scaly skin PMID: 20403327 
Tg(KRT14-cre)1Amc|Trpv3tm1.1Clph Tg(KRT14-cre)1Amc/0,Trpv3tm1.1Clph/Trpv3tm1.1Clph
involves: 129S4/SvJae * 129S4/SvJaeSor * C57BL/6 * C57BL/6J * CBA * Swiss Webster		 MGI:2181407  MGI:2450263  MP:0000410 waved hair PMID: 20403327 
Trpv3tm1.2Clph Trpv3tm1.2Clph/Trpv3tm1.2Clph
B6.129S4-Trpv3		 MGI:2181407  MP:0000410 waved hair PMID: 20403327 
Trpv3tm1.2Clph Trpv3tm1.2Clph/Trpv3tm1.2Clph
involves: 129S4/SvJae * 129S4/SvJaeSor * C57BL/6 * C57BL/6J * CBA		 MGI:2181407  MP:0000410 waved hair PMID: 20403327 
Clinically-Relevant Mutations and Pathophysiology
Disease:  Dermatitis and hairlessness.
References:  1
Click column headers to sort
Type Species Molecular location Description Reference
Missense Mouse G573C 1
Missense Mouse G573S 1

REFERENCES

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1. Asakawa, M; Yoshioka, T; Matsutani, T; Hikita, I; Suzuki, M; Oshima, I; Tsukahara, K; Arimura, A; Horikawa, T; Hirasawa, T; Sakata, T. (2006) Association of a mutation in TRPV3 with defective hair growth in rodents. J. Invest. Dermatol., 126 (12): 2664-72. [PMID:16858425]

2. Chung, MK; Guler, AD; Caterina, MJ. (2005) Biphasic currents evoked by chemical or thermal activation of the heat-gated ion channel, TRPV3. J. Biol. Chem., 280 (16): 15928-41. [PMID:15722340]

3. Chung, MK; Lee, H; Caterina, MJ. (2003) Warm temperatures activate TRPV4 in mouse 308 keratinocytes. J. Biol. Chem., 278 (34): 32037-46. [PMID:12783886]

4. Chung, MK; Lee, H; Mizuno, A; Suzuki, M; Caterina, MJ. (2004) 2-aminoethoxydiphenyl borate activates and sensitizes the heat-gated ion channel TRPV3. J. Neurosci., 24 (22): 5177-82. [PMID:15175387]

5. Macpherson, LJ; Hwang, SW; Miyamoto, T; Dubin, AE; Patapoutian, A; Story, GM. (2006) More than cool: promiscuous relationships of menthol and other sensory compounds. Mol. Cell. Neurosci., 32 (4): 335-43. [PMID:16829128]

6. Moqrich, A; Hwang, SW; Earley, TJ; Petrus, MJ; Murray, AN; Spencer, KS; Andahazy, M; Story, GM; Patapoutian, A. (2005) Impaired thermosensation in mice lacking TRPV3, a heat and camphor sensor in the skin. Science, 307 (5714): 1468-72. [PMID:15746429]

7. Peier, AM; Reeve, AJ; Andersson, DA; Moqrich, A; Earley, TJ; Hergarden, AC; Story, GM; Colley, S; Hogenesch, JB; McIntyre, P; Bevan, S; Patapoutian, A. (2002) A heat-sensitive TRP channel expressed in keratinocytes. Science, 296 (5575): 2046-9. [PMID:12016205]

8. Smart, D; Gunthorpe, MJ; Jerman, JC; Nasir, S; Gray, J; Muir, AI; Chambers, JK; Randall, AD; Davis, JB. (2000) The endogenous lipid anandamide is a full agonist at the human vanilloid receptor (hVR1). Br. J. Pharmacol., 129 (2): 227-30. [PMID:10694225]

9. Smith, GD; Gunthorpe, MJ; Kelsell, RE; Hayes, PD; Reilly, P; Facer, P; Wright, JE; Jerman, JC; Walhin, JP; Ooi, L; Egerton, J; Charles, KJ; Smart, D; Randall, AD; Anand, P; Davis, JB. (2002) TRPV3 is a temperature-sensitive vanilloid receptor-like protein. Nature, 418 (6894): 186-90. [PMID:12077606]

10. Xu, H; Delling, M; Jun, JC; Clapham, DE. (2006) Oregano, thyme and clove-derived flavors and skin sensitizers activate specific TRP channels. Nat. Neurosci., 9 (5): 628-35. [PMID:16617338]

11. Xu, H; Ramsey, IS; Kotecha, SA; Moran, MM; Chong, JA; Lawson, D; Ge, P; Lilly, J; Silos-Santiago, I; Xie, Y; DiStefano, PS; Curtis, R; Clapham, DE. (2002) TRPV3 is a calcium-permeable temperature-sensitive cation channel. Nature, 418 (6894): 181-6. [PMID:12077604]

To cite this database page, please use the following:

David E. Clapham, Bernd Nilius, Grzegorz Owsianik.
Transient Receptor Potential channels: TRPV3. Last modified on 01/03/2012. Accessed on 26/05/2013. IUPHAR database (IUPHAR-DB), http://iuphar-db.org/DATABASE/ObjectDisplayForward?objectId=509.


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