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RXFP2 receptor

Family: Relaxin family peptide receptors

Contents:
Gene and Protein Information
Previous and Unofficial Names
Database Links
Agonists
Antagonists
Transduction Mechanisms
Tissue Distribution
Expression Datasets
Functional Assays
Physiological Functions
Physiological Consequences of Altering Gene Expression
Phenotypes, Alleles and Disease Models
References
Gene and Protein Information
class A G protein-coupled receptor
Species TM AA Chromosomal Location Gene Symbol Gene Name Reference
Human 7 754 13q13.1 RXFP2 relaxin/insulin-like family peptide receptor 2 10
Mouse 7 737 5 G3 Rxfp2 relaxin/insulin-like family peptide receptor 2 15
Rat 7 737 12p12 Rxfp2 relaxin/insulin-like family peptide receptor 2 16
Previous and Unofficial Names
Leucine-rich repeat-containing G-protein coupled receptor
LGR8
RX2 receptor
INSL3 receptor
leucine-rich repeat-containing G protein-coupled receptor 8
GREAT
RX2
GPR106
INSL3R
RXFPR2
Gpcr
LOC363866
Lgr8_predicted
leucine-rich repeat-containing G protein-coupled receptor 8 (predicted)
relaxin receptor 2
relaxin/insulin-like family peptide receptor 2
Database Links
Ensembl
Entrez Gene
GeneCards
GenitoUrinary Development Molecular Anatomy Project
HomoloGene
Human Protein Reference Database
InterPro
KEGG Gene
OMIM
PharmGKB Gene
PhosphoSitePlus
Protein Ontology (PRO)
RefSeq Nucleotide
RefSeq Protein
TreeFam
UniGene Hs.
UniProt
Wikipedia
Search for 3D structures on the PDB
Search by keyword: Relaxin family peptide receptors RXFP2 receptor
Natural/Endogenous Ligand(s)
H1 relaxin {Sp: Human}
H2 relaxin {Sp: Human}
H3 relaxin {Sp: Human}
INSL3 {Sp: Human}
Rank order of potency (Human)
Agonists
Key to terms and symbols Click column headers to sort
Ligand Sp. Action Affinity Units Reference
[125I]INSL3 (human) Hs Full agonist 10.0 pKd 14
[33P]H2 relaxin Hs Full agonist 8.97 – 9.2 pKd 8,19
europium-labelled INSL3 Hs Full agonist 9.0 pKd 17
INSL3 {Sp: Human} Hs Full agonist 9.7 pKi 8
H1 relaxin {Sp: Human} Hs Full agonist 8.8 pKi 1
H2 relaxin {Sp: Human} Hs Full agonist 8.3 pKi 8
relaxin {Sp: Pig} Hs Full agonist 7.9 pKi 8
relaxin {Sp: Rhesus macaque} Hs Full agonist 7.8 pKi 8
H3 relaxin {Sp: Human} Hs Full agonist 7.0 pKi 1
Agonist Comments
Affinity values were determined in HEK 293 cells expressing human RXFP2.
Antagonist Comments
(des 1-8) A-chain INSL3 analog[2] and INSL3 B chain analog (Human)[3] are both selective antagonists for RXFP2.
Primary Transduction Mechanisms
Transducer Effector/Response
Gs family
Gi/Go family
Adenylate cyclase stimulation
Adenylate cyclase inhibition
References:  7-8,10-11,13,19
Tissue Distribution
Thyroid.
Species:  Human
Technique:  RT-PCR.
References:  9
Pituitary, testis, kidney.
Species:  Human
Technique:  RT-PCR.
References:  4
Testis, uterus, brain, kidney, thyroid, muscle, peripheral blood cell, bone marrow.
Species:  Human
Technique:  RT-PCR.
References:  10
Gubernaculum, testis and brain.
Species:  Mouse
Technique:  RT-PCR.
References:  15
Testis, ovary.
Species:  Rat
Technique:  Northern blot, in situ hybridisation.
References:  11
Kidney.
Species:  Rat
Technique:  RT-PCR, in situ hybridisation.
References:  5
Thalamus.
Species:  Rat
Technique:  RT-PCR, in situ hybridisation.
References:  18
Gubernaculum, testis.
Species:  Rat
Technique:  RT-PCR.
References:  12
Gubernaculum.
Species:  Rat
Technique:  Northern blot.
References:  13
Expression Datasets

Click here to show/hide data

Log average relative transcript abundance in mouse tissues measured by qPCR from Regard, J.B., Sato, I.T., and Coughlin, S.R. (2008). Anatomical profiling of G protein-coupled receptor expression. Cell, 135(3): 561-71. [PMID:18984166] [Raw data: website]

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Functional Assays
Measurement of cAMP levels in HEK 293T cells stably transfected with RXFP2 receptors.
Species:  Human
Tissue:  HEK 293T cells.
Response measured:  cAMP accumulation.
References:  7-8,10,13,19
Measurement of cAMP levels in cultured gubernacular cells.
Species:  Rat
Tissue:  Gubernaculum.
Response measured:  cAMP accumulation.
References:  13
Growth of organ cultures of day 17 fetal rat gubernaculum.
Species:  Rat
Tissue:  Fetal gubernaculum.
Response measured:  Growth by BrdU incorporation.
References:  12
Physiological Functions
Mediates the transabdominal phase of testicular descent.
Species:  Mouse
Tissue:  Gubernaculum.
References:  6
Oocyte maturation.
Species:  Rat
Tissue:  Oocyte.
References:  11
Male germ cell survival.
Species:  Rat
Tissue:  Male germ cells.
References:  11
Physiological Consequences of Altering Gene Expression
Male mice lacking the RXFP2 receptor demonstrate cryptorchidism due to the failure of the gubernaculum to develop.
Species:  Mouse
Tissue: 
Technique:  Gene targeting in embryonic stem cells.
References:  6
Phenotypes, Alleles and Disease Models Mouse data from MGI

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Allele Composition & genetic background Accession Phenotype Id Phenotype Reference
Rxfp2tm1Aia Rxfp2tm1Aia/Rxfp2tm1Aia
involves: 129S7/SvEvBrd * C57BL/6J
MGI:2153463  MP:0005149 abnormal gubernaculum morphology PMID: 12217959 
crsp|Rxfp2+|Rxfp2tm1Aia Rxfp2tm1Aia/crsp
involves: 129S7/SvEvBrd * C57BL/6J * FVB/N
MGI:1313307  MGI:2153463  MP:0005149 abnormal gubernaculum morphology PMID: 12217959 
Rxfp2tm1Aia Rxfp2tm1Aia/Rxfp2tm1Aia
involves: 129S7/SvEvBrd * C57BL/6J
MGI:2153463  MP:0003673 abnormal inguinal canal morphology PMID: 12217959 
Rxfp1tm1Aia|Rxfp2tm1Aia Rxfp1tm1Aia/Rxfp1tm1Aia,Rxfp2tm1Aia/Rxfp2tm1Aia
involves: 129S7/SvEvBrd * C57BL/6J
MGI:2153463  MGI:2682211  MP:0001882 abnormal lactation PMID: 15256493 
Rxfp1tm1Aia|Rxfp2tm1Aia Rxfp1tm1Aia/Rxfp1tm1Aia,Rxfp2tm1Aia/Rxfp2tm1Aia
involves: 129S7/SvEvBrd * C57BL/6J
MGI:2153463  MGI:2682211  MP:0006078 abnormal nipple morphology PMID: 15256493 
crsp|Rxfp2+|Rxfp2tm1Aia Rxfp2tm1Aia/crsp
involves: 129S7/SvEvBrd * C57BL/6J * FVB/N
MGI:1313307  MGI:2153463  MP:0009213 absent male inguinal canal PMID: 12217959 
Rxfp2tm1Aia Rxfp2tm1Aia/Rxfp2tm1Aia
involves: 129S7/SvEvBrd * C57BL/6J
MGI:2153463  MP:0001155 arrest of spermatogenesis PMID: 12217959 
crsp|Rxfp2+|Rxfp2tm1Aia Rxfp2tm1Aia/crsp
involves: 129S7/SvEvBrd * C57BL/6J * FVB/N
MGI:1313307  MGI:2153463  MP:0001155 arrest of spermatogenesis PMID: 12217959 
Rxfp2tm1Aia Rxfp2tm1Aia/Rxfp2tm1Aia
involves: 129S7/SvEvBrd * C57BL/6J
MGI:2153463  MP:0005159 azoospermia PMID: 12217959 
crsp|Rxfp2+|Rxfp2tm1Aia Rxfp2tm1Aia/crsp
involves: 129S7/SvEvBrd * C57BL/6J * FVB/N
MGI:1313307  MGI:2153463  MP:0005159 azoospermia PMID: 12217959 
Rxfp2tm1Aia Rxfp2tm1Aia/Rxfp2tm1Aia
involves: 129S7/SvEvBrd * C57BL/6J
MGI:2153463  MP:0002286 cryptorchism PMID: 12217959 
Rxfp1tm1Aia|Rxfp2tm1Aia Rxfp1tm1Aia/Rxfp1tm1Aia,Rxfp2tm1Aia/Rxfp2tm1Aia
involves: 129S7/SvEvBrd * C57BL/6J
MGI:2153463  MGI:2682211  MP:0002286 cryptorchism PMID: 15256493 
crsp|Rxfp2+|Rxfp2tm1Aia Rxfp2tm1Aia/crsp
involves: 129S7/SvEvBrd * C57BL/6J * FVB/N
MGI:1313307  MGI:2153463  MP:0002286 cryptorchism PMID: 12217959 
Rxfp2tm1Aia Rxfp2tm1Aia/Rxfp2tm1Aia
involves: 129S7/SvEvBrd * C57BL/6J
MGI:2153463  MP:0001925 male infertility PMID: 12217959 
crsp|Rxfp2+|Rxfp2tm1Aia Rxfp2tm1Aia/crsp
involves: 129S7/SvEvBrd * C57BL/6J * FVB/N
MGI:1313307  MGI:2153463  MP:0001925 male infertility PMID: 12217959 
Rxfp2tm1Aia Rxfp2tm1Aia/Rxfp2tm1Aia
involves: 129S7/SvEvBrd * C57BL/6J
MGI:2153463  MP:0001147 small testis PMID: 12217959 
crsp|Rxfp2+|Rxfp2tm1Aia Rxfp2tm1Aia/crsp
involves: 129S7/SvEvBrd * C57BL/6J * FVB/N
MGI:1313307  MGI:2153463  MP:0001147 small testis PMID: 12217959 

REFERENCES

To cite this database page, please use the following:

Ross Bathgate, Richard Ivell, Barbara Sanborn, David Sherwood, Roger Summers.
Relaxin family peptide receptors: RXFP2 receptor. Last modified on 15/02/2013. Accessed on 18/06/2013. IUPHAR database (IUPHAR-DB), http://iuphar-db.org/DATABASE/ObjectDisplayForward?objectId=3520.


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