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MRGPRF

Family: Class A Orphans

Contents:
Gene and Protein Information
Previous and Unofficial Names
Database Links
Agonists
Tissue Distribution
Gene Expression and Pathophysiology
Biologically Significant Variants
General Comments
References
Gene and Protein Information
class A G protein-coupled receptor
Species TM AA Chromosomal Location Gene Symbol Gene Name Reference
Human 7 343 11q13.2 MRGPRF MAS-related GPR, member F
Mouse 7 343 7 F5 Mrgprf MAS-related GPR, member F
Rat 7 343 1q42 Mrgprf MAS-related GPR, member F
Previous and Unofficial Names
GPR168
GPR140
RTA
MGC21621
mrgF
MAS-related GPR, member F
MRGPRF
MRGF
G protein-coupled receptor 168
G protein-coupled receptor 140
Mas-related gene F protein
G-protein coupled receptor 168
G-protein coupled receptor 14
mas-related G protein-coupled MRGF
seven transmembrane helix receptor
G protein-coupled receptor MrgF
mas-related G-protein coupled receptor member F
LOC266762
G protein coupled receptor (RTA)
G protein coupled receptor Rca
G-protein coupled receptor RTA
MAS-related G-protein coupled receptor, member F
MAS-related gene F
BC019711
Database Links
Ensembl ENSG00000172935 (Hs), ENSMUSG00000031070 (Mm), ENSRNOG00000013426 (Rn)
Entrez Gene 219928 (Hs), 211577 (Mm), 266762 (Rn)
GeneCards MRGPRF (Hs)
GenitoUrinary Development Molecular Anatomy Project Mrgprf (Mm)
HomoloGene 17023 (Hs)
Human Protein Reference Database 17590 (Hs)
InterPro Q96AM1 (Hs), Q8VCJ6 (Mm), P23749 (Rn)
KEGG Gene hsa:219928 (Hs), mmu:211577 (Mm), rno:266762 (Rn)
PhosphoSitePlus Q8VCJ6 (Mm), P23749 (Rn)
Protein Ontology (PRO) PRO:000001604 (Hs)
RefSeq Nucleotide NM_145015 (Hs), NM_145379 (Mm), NM_153722 (Rn)
RefSeq Protein NP_659452 (Hs), NP_663354 (Mm), NP_714944 (Rn)
TreeFam ENSG00000172935 (Hs), ENSMUSG00000031070 (Mm), ENSRNOG00000013426 (Rn)
UniGene Hs. 602397 (Hs)
UniProt Q96AM1 (Hs), Q8VCJ6 (Mm), P23749 (Rn)
Wikipedia MRGPRF
Orphan and other 7TM receptors
Agonist Comments
It is shown that MRGPRF responded to angiotensin metabolites stimulation [5].
Tissue Distribution
Illeum
Species:  Mouse
Technique:  RT-PCR
References:  1
Neuronal somata and nerve fibres in enteric plexuses (mysenteric neurons and submucosal neurons), nerve fibres in the tunica muscalaris and in the lamina propria
Species:  Mouse
Technique:  in situ hybridisation
References:  1
Highly expressed throughout large and small intestine, vas deferens, uterus, aorta and cerebellum. Barely detectable in liver, kidney, lung and salivary gland.
Species:  Rat
Technique:  Northern blot
References:  9
Tissue Distribution Comments
MRGPRF expression was not detected by in situ hybridisation in dorsal root ganglia [4].
Gene Expression and Pathophysiology Comments
MRGPRF is downregulated during intestinal schistisomiasis and ileitis [1].
Biologically Significant Variants
K16R
SNP accession:  rs11544721 
Type:  Naturally occurring SNPs.
Species:  Human
References: 
General Comments
It is found that Mrgprf expression was upregulated in Mrgpre knockout mice [3].

Of the eight human Mas-related GPCRs (MRGs), four (MRGPRD, MRGPRE, MRGPRF and MRGPRG) have clear orthologues in rodents, whereas the cluster of genes including human MRGPRX1, MRGPRX2, MRGPRX3 and MRGPRX4 is found only in primates and is replaced in rodents with a family of genes (>25 in mice, ~10 in rats) which have no obvious human counterparts [4]. Certain rodent MRGs have been reported to respond to adenine [2] and to RF-amide peptides including neuropeptide FF [6-7] but the relevance of these findings to man is unclear. MRGs are expressed predominantly in small diameter sensory neurons of the dorsal root ganglia, where there is emerging evidence that they may be mediators of histamine-independent itch [8,10].
Available Assays
DiscoveRx PathHunter® CHO-K1 MRGPRF (Orphan) High Expression β-Arrestin Cell Line Human Cat No. 93-0433C2A
DiscoveRx PathHunter® eXpress MRGPRF CHO-K1 β-Arrestin (Orphan) GPCR Assay Human Cat No. 93-0433E2ACP2

REFERENCES

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1. Avula, LR; Buckinx, R; Alpaerts, K; Costagliola, A; Adriaensen, D; Van Nassauw, L; Timmermans, JP. (2011) The effect of inflammation on the expression and distribution of the MAS-related gene receptors MrgE and MrgF in the murine ileum. Histochem. Cell Biol., 136 (5): 569-85. [PMID:21912971]

2. Bender, E; Buist, A; Jurzak, M; Langlois, X; Baggerman, G; Verhasselt, P; Ercken, M; Guo, HQ; Wintmolders, C; Van den Wyngaert, I; et al.. (2002) Characterization of an orphan G protein-coupled receptor localized in the dorsal root ganglia reveals adenine as a signaling molecule. Proc. Natl. Acad. Sci. U.S.A., 99 (13): 8573-8. [PMID:12084918]

3. Cox, PJ; Pitcher, T; Trim, SA; Bell, CH; Qin, W; Kinloch, RA. (2008) The effect of deletion of the orphan G - protein coupled receptor (GPCR) gene MrgE on pain-like behaviours in mice. Mol Pain, 4: 2. [PMID:18197975]

4. Dong, X; Han, S; Zylka, MJ; Simon, MI; Anderson, DJ. (2001) A diverse family of GPCRs expressed in specific subsets of nociceptive sensory neurons. Cell, 106 (5): 619-32. [PMID:11551509]

5. Gembardt, F; Grajewski, S; Vahl, M; Schultheiss, HP; Walther, T. (2008) Angiotensin metabolites can stimulate receptors of the Mas-related genes family. Mol. Cell. Biochem., 319 (1-2): 115-23. [PMID:18636314]

6. Han, SK; Dong, X; Hwang, JI; Zylka, MJ; Anderson, DJ; Simon, MI. (2002) Orphan G protein-coupled receptors MrgA1 and MrgC11 are distinctively activated by RF-amide-related peptides through the Galpha q/11 pathway. Proc. Natl. Acad. Sci. U.S.A., 99 (23): 14740-5. [PMID:12397184]

7. Lee, MG; Dong, X; Liu, Q; Patel, KN; Choi, OH; Vonakis, B; Undem, BJ. (2008) Agonists of the MAS-related gene (Mrgs) orphan receptors as novel mediators of mast cell-sensory nerve interactions. J. Immunol., 180 (4): 2251-5. [PMID:18250432]

8. Liu, Q; Tang, Z; Surdenikova, L; Kim, S; Patel, KN; Kim, A; Ru, F; Guan, Y; Weng, HJ; Geng, Y; Undem, BJ; Kollarik, M; Chen, ZF; Anderson, DJ; Dong, X. (2009) Sensory neuron-specific GPCR Mrgprs are itch receptors mediating chloroquine-induced pruritus. Cell, 139 (7): 1353-65. [PMID:20004959]

9. Ross, PC; Figler, RA; Corjay, MH; Barber, CM; Adam, N; Harcus, DR; Lynch, KR. (1990) RTA, a candidate G protein-coupled receptor: cloning, sequencing, and tissue distribution. Proc. Natl. Acad. Sci. U.S.A., 87 (8): 3052-6. [PMID:2109324]

10. Wilson, SR; Gerhold, KA; Bifolck-Fisher, A; Liu, Q; Patel, KN; Dong, X; Bautista, DM. (2011) TRPA1 is required for histamine-independent, Mas-related G protein-coupled receptor-mediated itch. Nat. Neurosci., 14 (5): 595-602. [PMID:21460831]

To cite this database page, please use the following:

Wen Chiy Liew.
Class A Orphans: MRGPRF. Last modified on 05/11/2012. Accessed on 25/05/2013. IUPHAR database (IUPHAR-DB), http://iuphar-db.org/DATABASE/ObjectDisplayForward?objectId=154.


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